METHODOLOGY REPORTS

Antibodies:

 

Deb, S., Hashizume, K., Boone, K., Southard, J.N., Talamantes, F., Rawitch, A. and Soares, M.J. (1989) Antipeptide antibodies reveal structural and func­tional characteristics of rat placental lactogen‑II. Molecular & Cellular Endocrinology 63: 45‑56.

 

Deb, S., Youngblood, T., Rawitch, A. and Soares, M.J. (1989) Placental prolactin‑like protein‑A: identification and characterization of two major glycoprotein species with antipeptide antibodies. Journal of Biological Chem­istry 264: 14348‑14353.

 

Roby, K.F., Larsen, D., Deb, S., and Soares, M.J. (1991) Generation and characterization of antipeptide antibodies to rat cytochrome P-450 side chain cleavage enzyme.  Molecular & Cellular Endocrinology 79: 13-20.

 

Deb, S., Faria, T.N., Roby, K.F., Larsen, D., Kwok, S.C.M., Talamantes, F. and Soares, M.J. (1991) Identification and characterization of a new member of the placental prolactin family: placental lactogen-I variant.  Journal of Biological Chemistry 266: 1605-1610.

 

Campbell, W.J., Larsen, D., Deb., S., Kwok, S.C.M. and Soares, M.J.  (1991) Expression of alkaline phosphatase in differentiated rat labyrinthine trophoblast tissue.  Placenta 12: 227-237.

 

Deb, S., Roby, K.F., Faria, T.N., Larsen, D., and Soares, M.J. (1991) Identification and immunochemical characterization of a major placental secretory protein related to the prolactin-growth hormone family, prolactin-like protein-C.  Endocrinology 128: 3066-3072.

 

Deb, S., Hamlin, G.P., Kwok, S.C.M., and Soares, M.J. (1993) Heterologous expression and characterization of prolactin-like protein-A: identification of serum binding proteins.  Journal of Biological Chemistry 268: 3298-3305.

 

Rasmussen, C.A., Hashizume, K., Orwig, K.E., Xu, L., and Soares, M.J.  (1996) Decidual prolactin-related protein:  heterologous expression and characterization.  Endocrinology 137: 5558-5566

 

Cohick, C.B., Xu, L., and Soares, M.J. (1997) Placental prolactin-like protein-B:  heterologous expression and characterization of placental and decidual species.   Journal of Endocrinology 152: 291-302

 

 

Cell Models:

Sahgal, N., Canham, L.N., Canham, B., and Soares, M.J. (2006) Rcho-1 trophoblast cells:  a model for studying trophoblast differentiation. Methods in Molecular Medicine 121: 159-178.

 

Asanoma, K., Rumi, M.A.K., Kent, L.N., Chakraborty, D., Renaud, S.J., Wake, N., Lee, D.-S., Kubota, K., and Soares, M.J. (2011) FGF4-dependent stem cells derived from rat blastocysts differentiate along the trophoblast lineage.  Developmental Biology 351: 110-119.

 

 

Methods/Protocols:

Ain, R., Tash, J.S., and Soares, M.J. (2002) A simple method for the in situ detection of eosinophils. Journal of Immunological Methods 260: 273-278

 

Arroyo, J.A., Konno, T., Khalili, D., and Soares, M.J. (2005) A simple in vivo approach to investigate invasive trophoblast cells.  International Journal of Developmental Biology 49: 977-980.

 

Sahgal, N., Canham, L.N., Canham, B., and Soares, M.J. (2006) Rcho-1 trophoblast cells:  a model for studying trophoblast differentiation. Methods in Molecular Medicine 121: 159-178.

 

Ain, R., Konno, T., Canham, L.N., and Soares, M.J. (2006) Phenotypic analysis of the placenta in the rat.Methods in Molecular Medicine 121: 295-313.

 

Müller, H. and Soares, M.J. (2006) Alkaline phosphatase fusion proteins as tags for identifying targets for placental ligands. Methods in Molecular Medicine 122: 331-340.

 

Konno, T., Rempel, L.A., Arroyo, J.A., and Soares, M.J. (2007) Pregnancy in the Brown Norway rat:  a model for investigating the genetics of placentation.  Biology of Reproduction 76: 709-718. 

 

Dhakal, P. and Soares, M.J. (2017) Single-step PCR-based genetic sex determination of rat tissues and cells.  BioTechniques 62: 232-233.

 

Chakraborty, D., Muto, M., and Soares, M.J. (2017) Ex vivo lentiviral trophoblast-specific shRNA delivery and analysis of placental phenotypes.  Bio-Protocol 7: e2652.

 

Chakraborty, D., Scott, R.L., and Soares, M.J. (2018) Hypoxia signaling and placental adaptations. Methods in Molecular Biology 1742: 167-183.

The Institute for Reproduction and Perinatal Research 

The University of Kansas Medical Center

© 2018 by Soares Lab Members- Proudly created with Wix.com