
Institute for Reproductive and Developmental Sciences
The University of Kansas Medical Center, Kansas City, KS
METHODOLOGY REPORTS
Antibodies:
Deb, S., Hashizume, K., Boone, K., Southard, J.N., Talamantes, F., Rawitch, A. and Soares, M.J. (1989) Antipeptide antibodies reveal structural and functional characteristics of rat placental lactogen‑II. Molecular & Cellular Endocrinology 63: 45‑56.
Deb, S., Youngblood, T., Rawitch, A. and Soares, M.J. (1989) Placental prolactin‑like protein‑A: identification and characterization of two major glycoprotein species with antipeptide antibodies. Journal of Biological Chemistry 264: 14348‑14353.
Roby, K.F., Larsen, D., Deb, S., and Soares, M.J. (1991) Generation and characterization of antipeptide antibodies to rat cytochrome P-450 side chain cleavage enzyme. Molecular & Cellular Endocrinology 79: 13-20.
Deb, S., Faria, T.N., Roby, K.F., Larsen, D., Kwok, S.C.M., Talamantes, F. and Soares, M.J. (1991) Identification and characterization of a new member of the placental prolactin family: placental lactogen-I variant. Journal of Biological Chemistry 266: 1605-1610.
Campbell, W.J., Larsen, D., Deb., S., Kwok, S.C.M. and Soares, M.J. (1991) Expression of alkaline phosphatase in differentiated rat labyrinthine trophoblast tissue. Placenta 12: 227-237.
Deb, S., Roby, K.F., Faria, T.N., Larsen, D., and Soares, M.J. (1991) Identification and immunochemical characterization of a major placental secretory protein related to the prolactin-growth hormone family, prolactin-like protein-C. Endocrinology 128: 3066-3072.
Deb, S., Hamlin, G.P., Kwok, S.C.M., and Soares, M.J. (1993) Heterologous expression and characterization of prolactin-like protein-A: identification of serum binding proteins. Journal of Biological Chemistry 268: 3298-3305.
Rasmussen, C.A., Hashizume, K., Orwig, K.E., Xu, L., and Soares, M.J. (1996) Decidual prolactin-related protein: heterologous expression and characterization. Endocrinology 137: 5558-5566
Cohick, C.B., Xu, L., and Soares, M.J. (1997) Placental prolactin-like protein-B: heterologous expression and characterization of placental and decidual species. Journal of Endocrinology 152: 291-302
Cell Models:
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Sahgal, N., Canham, L.N., Canham, B., and Soares, M.J. (2006) Rcho-1 trophoblast cells: a model for studying trophoblast differentiation. Methods in Molecular Medicine 121: 159-178.
Asanoma, K., Rumi, M.A.K., Kent, L.N., Chakraborty, D., Renaud, S.J., Wake, N., Lee, D.-S., Kubota, K., and Soares, M.J. (2011) FGF4-dependent stem cells derived from rat blastocysts differentiate along the trophoblast lineage. Developmental Biology 351: 110-119.
Methods/Protocols:
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Ain, R., Tash, J.S., and Soares, M.J. (2002) A simple method for the in situ detection of eosinophils. Journal of Immunological Methods 260: 273-278
Arroyo, J.A., Konno, T., Khalili, D., and Soares, M.J. (2005) A simple in vivo approach to investigate invasive trophoblast cells. International Journal of Developmental Biology 49: 977-980.
Sahgal, N., Canham, L.N., Canham, B., and Soares, M.J. (2006) Rcho-1 trophoblast cells: a model for studying trophoblast differentiation. Methods in Molecular Medicine 121: 159-178.
Ain, R., Konno, T., Canham, L.N., and Soares, M.J. (2006) Phenotypic analysis of the placenta in the rat.Methods in Molecular Medicine 121: 295-313.
Müller, H. and Soares, M.J. (2006) Alkaline phosphatase fusion proteins as tags for identifying targets for placental ligands. Methods in Molecular Medicine 122: 331-340.
Konno, T., Rempel, L.A., Arroyo, J.A., and Soares, M.J. (2007) Pregnancy in the Brown Norway rat: a model for investigating the genetics of placentation. Biology of Reproduction 76: 709-718.
Dhakal, P. and Soares, M.J. (2017) Single-step PCR-based genetic sex determination of rat tissues and cells. BioTechniques 62: 232-233.
Chakraborty, D., Muto, M., and Soares, M.J. (2017) Ex vivo lentiviral trophoblast-specific shRNA delivery and analysis of placental phenotypes. Bio-Protocol 7: e2652.
Chakraborty, D., Scott, R.L., and Soares, M.J. (2018) Hypoxia signaling and placental adaptations. Methods in Molecular Biology 1742: 167-183.